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News: Welcome to the cell culture forum set up in association with 'Human Embryonic Stem Cells: The Practical Handbook'. This resource has been set up to allow users of our book to communicate with each other using themed discussion boards. It is our hope that this will stimulate debate and facilitate the exchange of ideas and practical tips within the human ES cell research community. All that is required is that you sign up as a member of the forum. JUST CLICK ON THE REGISTER LINK ABOVE TO GET STARTED.

1   Cell Culture Forum / New publications and insights / The American Medical Association praises HES: The practical handbook  on: September 04, 2008, 04:45:56 AM  Started by stephensull | Last post by stephensull JAMA. 2008;300(9):1082 (doi:10.1001/jama.300.9.1082) Christopher M. Walsh Human Embryonic Stem Cells: The Practical Handbook "...investigations in turn have led to the absence of standardized protocols in human embryonic stem cell research and the parallel development of protocols to reach a common end. Enter Human Embryonic Stem Cells: The Practical Handbook. This laboratory handbook serves the valuable purposes of being a dense trove of protocols and advice for the seasoned or novice human embryonic stem cell researcher and of drawing the research community together by presenting the vast variations among its protocols." "Handbook is a valuable resource for seasoned and novice researchers" The AMA also praised the website: "An added bonus to the hard copy handbook is a discussion forum at http://www.HumanESCellBook.com, where registered participants can openly post questions and engage in discussions of topics such as protocols and techniques."  2   Cell Culture Forum / Directed Differentiation of Human ES Cells / D'Amour Protocol for Insulin producing cells  on: March 21, 2008, 01:58:50 PM  Started by jgiraldo | Last post by jgiraldo I'm trying to do the protocol without feeders, but have a lot of cell death once I start it. The suggestion is to add bFGF (FGF2).. but how much? 100ng/ml is used too expand cells in an undifferentiated state, and 4-10ng/ml is used in CM.  Has anybody had the same problem and how was this dealt with?  3   Cell Culture Forum / Ethics and Politics Pretaining to Human ES Cells / Re: sorting the second hand sales men from the bona fide stem cell researchers  on: October 10, 2007, 10:32:59 PM  Started by robh | Last post by robh http://www.hsci.harvard.edu/node/45 Here is a good talk for lay people to get a rough idea where stem cell research is at currently.  4   Cell Culture Forum / Ethics and Politics Pretaining to Human ES Cells / Re: sorting the second hand sales men from the bona fide stem cell researchers  on: October 10, 2007, 10:20:02 PM  Started by robh | Last post by stephensull Yes, I agree this is disturbing.....there are many such dubious 'stem cell therapy' centres around the world, in Russia, Czech Republic, India and elsewhere. It is important that we convey the difference between uncontroled and controlled research to the greater public. It seems obvious to experts in the field which sources are trustworthy and which aren't, unfortunately both can post a web site and attract attention. We had better do more collectively to tackle this growing problem through education or we shall all suffer.  5   Cell Culture Forum / Ethics and Politics Pretaining to Human ES Cells / sorting the second hand sales men from the bona fide stem cell researchers  on: October 10, 2007, 10:05:44 PM  Started by robh | Last post by robh http://news.bbc.co.uk/2/hi/programmes/documentary_archive/7035462.stm I just listened to this program on the BBC about maverick stem cell cures and the 'clinicians' who provide these completely unsubstantiated treatments. It illustrates the need for educating the public about stem cell research and the importance of supporting high quality accurately controlled research. The desparate position of patients should not be taken advantage of by certain self serving or genuinely misguided individuals. Rob H  6   Cell Culture Forum / Ethics and Politics Pretaining to Human ES Cells / Questions human embryonic stem cell researchers are commonly asked in the media  on: October 03, 2007, 08:45:48 AM  Started by stephensullivan | Last post by stephensullivan Here is a list of questions I was asked recently by an Irish TV production company making a documentary on embryonic stem cell research. I post it as I think it represents the majority of questions the media tend to ask stem cell researchers. Science What is a stem cell? What’s so good about stem cells? Where do they come from? How do scientists get them? How do scientists use them? What is an embryo? What size is a single embryo – example (how many would fit on a pinhead/tip of a biro)? What’s the difference between an embryo and a foetus? What is an embryonic stem cell? What is the difference between adult stem cells and embryonic stem cells? What is the most important characteristic of embryonic stem cells? Are embryonic stem cells better than adult stem cells? IVF What is your personal view of IVF treatment? What’s your view on the surplus embryos that are created as a result of IVF? What happens to embryos in storage, do they last forever? Why should people donate their embryos for research? How do you feel about couples who donate surplus embryos for research? What would you say to Irish couples who have surplus embryos in storage? Research Why are scientists getting excited about stem cell research? What are the potential benefits of stem cell research? Which conditions / diseases might stem cell research / therapy help? How can stem cells be used to investigate the causes of / find cures for diseases? Describe the significance of the discovery of the stem cell for society at large. What are the big challenges in stem cell research? Some people argue that just as many benefits can be taken from non-embryonic stem cells – what’s your opinion on this? What are the big ESC research ‘success stories’ in the last few years? People are expecting all sorts of benefits just around the corner, what’s the reality? What is the timescale for new and effective treatments using stem cells ? Why does it take so long to deliver the goods? Is it all overhyped? Are there any stem cell treatments currently available? What’s your opinion of the so-called ESC ‘therapies’ on offer in countries like the Ukraine? The desperate patients travelling for these treatments – what would you say to them? Harvard What is the Harvard Stem Cell Institute? What happens here? What work are you doing now? Where do you get the stem cell lines you work with here? How will this help to cure diseases? Could you do this work in Ireland? Would you like to do this work in Ireland? Debate Is an embryo a human life? There’s a huge amount of debate over whether an embryo represents ‘life’ – what’s you position on this? How do you feel about the use of embryos for stem cell research? What is the situation with using IVF surplus embryos for ESC in the US? Why is it so difficult for scientists to agree on a definition of what constitutes human life / when it begins – and give us an answer we can use? How can we reconcile the opposing views on these issues? Cloning What about ‘cloning’ - where does that fit into stem cell research? Can you explain in very simple terms the difference between a cloned embryo and a non-cloned one? Explain the difference between theutic and reproductive cloning Do you have an opinion on the use of theutic cloning as a source of embryos for stem cell research? For many people, cloning conjures up sci- fi images of using bodies for spare parts, a clone underclass and the possibility of ‘eternal life’ – what’s your take on this? Can you tell me what hybrid cloning is? What concerns do people have about this technology? What’s your reaction to these concerns? The Future Where do you see the future for embryonic stem cell research and treatments? What do you believe will happen in Ireland? What should we be doing in Ireland to move the situation on? Some argue that, if we don’t accept ESC research/therapy in Ireland, then Irish people will simply go abroad for treatment – what’s your take on that? What would be your ideal situation in Ireland: for the options for dealing with surplus embryos? for stem cell research? for regulation?  7   Cell Culture Forum / New publications and insights / Re: what happens to a differentiated cell if implanted back into the blastocyst  on: September 18, 2007, 06:52:39 AM  Started by justin | Last post by stephensullivan Some early work was done with mouse melanocytes and showed that reimplanted melanocytes could contribute within their own niche, looked like the cells presisted until the correct developmental time, rather than dedifferentiated. I think this was a Karl Illmensee paper. Will check. More recently Rudolf Jaenisch readdressed this issue by implanting cancer cells into mouse blastocysts, most of the cell lines he used didnt contribute to the chimera, but melonoma cells seem to have dedifferentiated and contributed.  8   Cell Culture Forum / Directed Differentiation of Human ES Cells / What are the cells types that can readily be derived from human ES cells now?  on: September 17, 2007, 07:06:37 PM  Started by paolo | Last post by paolo I know generally it seems to be more difficult to make lineages of the endoderm rather than the other two germ layers, but what are the cells we can routinely differentiate into now?  9   Cell Culture Forum / Ethics and Politics Pretaining to Human ES Cells / Re: Moral Courage: the responsiblity of scientists  on: September 17, 2007, 06:59:41 PM  Started by stanlie1981 | Last post by justin People are most lightly to be afraid of that which they no little or nothing about. I am from Hawaii, and there, where we invented mouse cloning we have a positive perseption of this type of research. If they didnt, I would feel oligated to discuss my research anyway. We must all shine a light on this research.  10   Cell Culture Forum / New publications and insights / What happens to a differentiated cell if implanted back into the blastocyst  on: September 17, 2007, 06:53:08 PM  Started by justin | Last post by justin If you insert a differentiated somatic cell into the inner cell mass of the blastocyst, will this cell undergo reprogramming or contribute to the embryo? I know this is an unethical experiment in human context, just wanted to now what happens in mouse/other species? Return to the board index.

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