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News: Welcome to the cell culture forum set up in association with 'Human Embryonic Stem Cells: The Practical Handbook'. This resource has been set up to allow users of our book to communicate with each other using themed discussion boards. It is our hope that this will stimulate debate and facilitate the exchange of ideas and practical tips within the human ES cell research community. All that is required is that you sign up as a member of the forum. JUST CLICK ON THE REGISTER LINK ABOVE TO GET STARTED.

Cell Culture Forum   Cell Culture Forum   Routine Culture of Human ES Cells   What are the main things veterans can teach those new to hES cell culture? « previous next » Pages: [1] Author Topic: What are the main things veterans can teach those new to hES cell culture?  (Read 1602 times) justin Newbie Posts: 5 What are the main things veterans can teach those new to hES cell culture? « on: September 17, 2007, 06:27:20 PM » I have just started human ES cell culture. What advice do veterans have for me? What did you learn that you wish you known at the begining? Report to moderator   Logged stephensullivan Administrator Newbie Posts: 6 Re: What are the main things veterans can teach those new to hES cell culture? « Reply #1 on: September 17, 2007, 06:36:56 PM » Don't expect the cells to behave like their mouse counterparts. You are definately better off not using antibiotics constitutively. A superbug will eventually arise and you will have no way of fighting it. Use high quality (low passage, unstressed looking) feeders at a concentration greater than 5,000 cells/cm2. Expect the cells to grow slow when seeded at low confluence. To increase cell number fast, resist the temptation to split the hES cells greater than 1:4. Get your stock cells from a good source and verify they are mycoplasma free before you start expanding and using them routinely. If you are worried about potenial contamination, grow your cultures in flasks rather than plates. « Last Edit: October 03, 2007, 11:00:11 AM by stephensullivan » Report to moderator   Logged Pages: [1]   « previous next » Jump to: Please select a destination: ----------------------------- Cell Culture Forum ----------------------------- => New publications and insights => Routine Culture of Human ES Cells => Characterizing Human ES Cells => Genetic Manipulation of Human ES Cells => Directed Differentiation of Human ES Cells => Ethics and Politics Pretaining to Human ES Cells

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