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News: Welcome to the cell culture forum set up in association with 'Human Embryonic Stem Cells: The Practical Handbook'. This resource has been set up to allow users of our book to communicate with each other using themed discussion boards. It is our hope that this will stimulate debate and facilitate the exchange of ideas and practical tips within the human ES cell research community. All that is required is that you sign up as a member of the forum. JUST CLICK ON THE REGISTER LINK ABOVE TO GET STARTED.

Cell Culture Forum   Cell Culture Forum   Directed Differentiation of Human ES Cells   D'Amour Protocol for Insulin producing cells « previous next » Pages: [1] Author Topic: D'Amour Protocol for Insulin producing cells  (Read 1399 times) jgiraldo Newbie Posts: 1 D'Amour Protocol for Insulin producing cells « on: March 21, 2008, 01:58:50 PM » I'm trying to do the protocol without feeders, but have a lot of cell death once I start it. The suggestion is to add bFGF (FGF2).. but how much? 100ng/ml is used too expand cells in an undifferentiated state, and 4-10ng/ml is used in CM.  Has anybody had the same problem and how was this dealt with? Report to moderator   Logged Pages: [1]   « previous next » Jump to: Please select a destination: ----------------------------- Cell Culture Forum ----------------------------- => New publications and insights => Routine Culture of Human ES Cells => Characterizing Human ES Cells => Genetic Manipulation of Human ES Cells => Directed Differentiation of Human ES Cells => Ethics and Politics Pretaining to Human ES Cells

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